Prevalence of blaACC and blaMOX genes in Klebsiella pneumonia isolated from Al-Rumetha hospital in Al-Muthanna Province, Iraq

Abstract

Ampicillin hydrolyzing class C β-lactamase (AmpC) that confer resistance to extended spectrum cephalosporins and never inhibited by β- lactamase inhibitors. Results of this study confirmed that 37 (41.1%) isolates of Klebsiella pneumonia from 90 isolates tested for cefoxitin susceptibility by disk diffusion method. AmpC β-lactamase producers were obtained from clinical isolates; 13 (35.1%) from urine, 10 (27%) from blood, 10 (27%) from wound, and 4 (10.8%) from vagina. AmpC β-lactamase producer isolates were confirmed in for cefoxitin resistance 37 K. pneumoniae isolates by modified three dimensional test and AmpC disk test. From 90 isolates, plasmid encoded AmpC genes were detected by multiplex PCR in 30 (33.3%) of the K. pneumoniae isolates. Of these, plasmid-encoded AmpC genes belonging to the MOX family were detected in 14/30 (46.7 %) isolates. Gene of the family ACC type was present in 2/30 (6.7%) isolates. While, the other isolates 14/30 (46.7%): 7(23.3%), 4(13.3%), 2(6.7%) and 1(3.3%), isolates contained another families like DHA, FOX, CIT and EBC, respectively.