Coating urinary catheter with moxifloxacin restricts Stenotrophomonas maltophilia adhesion in vitro

Abstract

Ability of Stenotrophomonas maltophilia to adhere and form biofilm onto abiotic surfaces is one of important features of clinical and environmental isolates. Ability of S. maltophilia to adhere to urinary catheter in vitro was not evaluated clearly. Here, the efficiency of S. maltophilia to adhere to coated and uncoated urinary catheter with moxifloxacin was evaluated for first time. Pieces of catheters were coated with moxifloxacin. Spectrophotometric method was used to check the moxifloxacin leached onto urinary catheter. Coated and uncoated catheters were incubated with bacterial growth. Viable of bacterial count was used to check the number of adhered bacteria onto both catheters. Significant adhesion of S. maltophilia to urinary catheter (uncoated with moxifloxacin) in vitro was started as early as 15 min post incubation with bacterial suspension (107 c.f.u./ml). Maximum adhesion was observed at 48 h. Pretreatment of urinary catheter in vitro with 50 ug of moxifloxacin per ml for 24 h significantly reduced the adhesion and survival of the clinical isolate S. maltophilia (Sm2). The significant reduce (P < 0.05) of bacterial adhesion was found at each time point (1, 4, 24 h). The current study showed for the first time high efficiency of S. maltophilia to adhere to urinary catheter in vitro. Moreover, the present study demonstrated for the first time that the coated catheter with moxifloxacin reduced significantly ability of S. maltophilia adhesion and biofilm formation in vitro.