Hypoglycemic Effect of 24-Methylencycloartan-3-one Isolated from Prosopis juliflora Pods in Alloxan Induced Diabetic Rabbits

Abstract

Prosopis juliflora is herbal plant uses in different countries to treat diabetes mellitus. In current study, oil extract was extracted from P. juliflora pods. Qualitative preliminary tests showed the extract contained terpenoids. The oil compound was isolated purified and identified by thin layer chromatography (TLC), gas chromatography-mass (GC-MS), nuclear magnetic resonance (H1-NMR and C13-NMR) and infra red (IR) spectroscopy. It was found that oils extract contain 24-Methylencycloartan-3-one. Hypoglycemic effect for this compound was checked in alloxan induced fasted diabetic rabbits. The dose of 0.3 gm/kg of terpenoidic at different time intervals (2,4,6 and 24 h) reduced blood glucose significantly with values 321.42, 294.60, 251.40 and 172.30 mg /100 ml, respectively. The toxicity study has shown that the 24-Methyl cycloartan compound has no toxic effect on red blood cells; therefore we suggest that this compound can be successfully and safely use to treat diabetes mellitus instead of insulin