Production, optimization, and characterization of cellulose produced from Pseudomonas spp

Abstract

Bacterial cellulose is a type of biopolymer produced by many Gram-negative bacteria such as Acetobacter, Azotobacter, Rhizobium, Pseudomonas, Salmonella, Alcaligenes, and Gram positive bacteria such as Sarcina ventriculi. Bacterial cellulose characterized by high purity containing no hemicellulose or lignin, high water holding capacity and hydrophilicity, good mechanical strength, elasticity and high crystallinity. This article aimed to study the optimum conditions for the production and extraction of cellulose produced from different species of Pseudomonas isolated from different samples of food. The results showed that the best production was obtained in cellulose production medium (HS- medium) containing 2% dates molasses, 1% yeast extract, pH 5, inoculated with 10% of bacterial culture and incubated at 30 0C for one week in shaker incubator. Fourier transform infrared spectroscopy (FTIR) analysis for Pseudomonas f3 cellulose showed peaks at 3365.55 cm-1, 2968.24 cm-1, 2852.52 cm-1, 1118.64 cm-1 and 1436.87 cm-1. These peaks indicating the presence of stretching O-H, C-H, H-C-H, C-O-C, and C-C, which identifying functional groups present in Pseudomonas f3 cellulose.