The effect of Nd: YAG lasers on Leishmania donovani promastigotes

Authors

  • Hanna S. Sabaa Department of Physics, College of Science, Al- Mustansiriya University, Baghdad, Iraq Author
  • Khawla H. Zghair Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq Author
  • Nebras R. Mohammed Department of Biology, College of Science, Al- Mustansiriya University, Baghdad, Iraq Author
  • Israa S. Musa Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq Author
  • Raad S. Abd Department of Physics, College of Science, Al- Mustansiriya University, Baghdad, Iraq. Author

Keywords:

Attenuated promastigotes, Leishmania donovani, Nd: YAG laser

Abstract

The present study was conducted to assess the effect of Nd:YAG laser on Leishmania donovani promastigotes. L. donovani promastigotes exposed to Nd:YAG laser (wavelength 1060 A°) in 500, 1000 and 1500 pulse between each pulse and another 6 seconds in comparison to the control (L. donovani promastigotes without exposure to Nd:YAG laser. The effect of Nd:YAG laser on the viability of L. donovani promastigotes was measured using the colorimetric MTT assay and the percentage of viability was counted. L. donovani viable cells were decreased with long exposure to Nd-YAG laser. Nd:YAG laser was effective in killing most of L. donovani promastigotes, and the remaining were devoid of flagellum that may lost their ability to cause infection.

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Published

2016-01-10

Issue

Volume 4 Number 1 (2016)

Section

Research Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Sabaa, H. S., Zghair, K. H., Mohammed, N. R., Musa, I. S., & Abd, R. S. (2016). The effect of Nd: YAG lasers on Leishmania donovani promastigotes. World Journal of Experimental Biosciences (ISSN: 2313-3937) , 4(1), 25-28. https://wjebio.com/index.php/journal/article/view/75