Effect of combination of D-glycin and antibiotics on biofilm formation by clinical, food and environmental isolates of Escherichia coli

Authors

  • Marwa H. M. Al-Khafaji Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq Author
  • Heba K. Tawfeeq Central Environmental Laboratory, College of Science, Baghdad University, Baghdad, Iraq. Author
  • Beadaa A. Mahdii Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq Author

Keywords:

Biofilm, Ceftriaxone, D-glycin, Escherichia coli, Imipenem

Abstract

The ability of many bacteria to adhere to surfaces and form biofilm makes the treatment of infections so difficult and has major consequences in a variety of industries such as food industry. Biofilm generates a persistent cause of contamination, thus, in the present study; inhibition of biofilm formation efficacy of the combination of D-glycin with antibiotics was compared with the antibiotic alone against Escherichia coli biofilm formation. It was found that the synergetic action of antibiotics with D-glycin was effective on inhibition of biofilm formation. The isolates ability to form biofilm was assayed using the tissue culture plate and Congo-red agar methods and findings of this study were source related; since food origin isolates were more biofilm producers when the Congo-red agar was used as compared with tissue culture plate method. Minimum inhibitory concentration (MIC) of D-glycin, imipenem and ceftriaxone were determined. The combinations of D-glycin with antibiotics inhibited bacterial biofilm formation more than the antibiotics imipenem and ceftriaxone separately. Susceptibility test to eight antibiotics: Imipenem, ceftriaxone, ampicillin, amoxicillin, cephalexin, clarithromycin, gentamycin and tetracycline; was performed on planktonic cells, and the cells exhibited different sensitivity patterns to them depending on their source.

 

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Published

2016-08-12

Issue

Section

Research Article

How to Cite

Al-Khafaji, M. H. M., Tawfeeq, H. K., & Mahdii, B. A. (2016). Effect of combination of D-glycin and antibiotics on biofilm formation by clinical, food and environmental isolates of Escherichia coli. World Journal of Experimental Biosciences (ISSN: 2313-3937) , 4(2), 112-117. https://wjebio.com/index.php/journal/article/view/96